Flow Cytometry, Leukemia/Lymphoma/Myeloma
GENERAL INFORMATION
Test Name:
Flow Cytometry, Leukemia/Lymphoma/Myeloma
PC/NPS Test Code:
FLOW LEUK
CPT Code(s):
88184 (first antibody) + 88185 for each additional antibody + 88187 (interp for 2-8 antibodies) or 88188 (interp for 9-15 antibodies) or 88189 (interp for >15 antibodies)
Methodology:
Flow cytometry
Days of Week Performed:
M, Tu, W, Th, F
Routine TAT:
1-2 days (excluding weekends and holidays)
COLLECTION AND HANDLING:
Collection Instructions:
BLOOD: One lavender (EDTA) tube. Lymph Node Tissue placed in RPMI media (available from Path Consultants). Stable in RPMI media 6 hours at room temp or 24 hours refrigerated. If RPMI media is not available, sterile saline may be used. BODY FLUIDS: Including pleural fluid, BAL, CSF and vitreous fluid. Submit to the lab ASAP. For low cellularity samples prone to rapid cell lysis such as CSF and vitreous fluid, mix in approximately equal volumes with RPMI media and refrigerate if testing will not be performed the same day. Specimens which have had fixatives added (e.g., formalin or PAP fixative) cannot be used for flow cytometry. BONE MARROW: Transfer 1-3 mL into a lavender tube (EDTA), or directly into a tube of RPMI media. Green (heparin) or ACD (yellow) are also acceptable. A bone marrow biopsy placed in RPMI media or saline can be used with pathologist approval. Prepare bone marrow slides from the fresh specimen, especially if the specimen will not be tested the same day. Also include blood films and a copy of the CBC if available.
Collection Containers:
Varies by specimen type. See collection instructions above.
Specimen Transport Requirements:
Refrigerate if testing will not be performed within 1-2 hours.
Specimen Labeling:
Label containers and slides with two patient specific identifiers.
Rejection Criteria:
Clotting. Freezing. Specimen age. Incorrect specimen type. Insufficient cells for analysis. Poor cell viablity. NOTE: For irreplaceable samples (bone marrow, tissues and body fluids), testing will be performed on sub-optimal samples whenever possible. A pathologist will then decide whether to report results with a disclaimer, or have the testing cancelled.
